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Recording Multicellular Behavior in Myxococcus xanthus Biofilms using Time-lapse Microcinematography
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Journal JoVE Biologie
Recording Multicellular Behavior in Myxococcus xanthus Biofilms using Time-lapse Microcinematography

Recording Multicellular Behavior in Myxococcus xanthus Biofilms using Time-lapse Microcinematography

DOI:
10.3791/2038-v

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10:59 min

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August 06, 2010

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,
1Department of Environmental Health Sciences,University of South Carolina (USC), 2Department of Biology,Syracuse University

Chapitres

  • 00:04Titre
  • 00:49Introduction
  • 01:11Preparing Bacteria and Nutritive Disk
  • 02:59Setting Up Plate Complexes
  • 06:19Plating Cells and Assembling Assay
  • 07:56Preparing the Movie
  • 08:45Time-Lapse Microcinematography
  • 10:35Conclusion

Summary

Traduction automatique

Traduction automatique

To study Myxococcus xanthus swarm behavior, we have designed a time-lapse microcinematography protocol that can be modified for different assays. It employs standard growth conditions adapted for microscopy, and yields reproducible results by the use of inexpensive, reusable silicone gaskets. We have used this method to quantify multicellular chemotaxis.

Tags

Multicellular BehaviorMyxococcus XanthusBiofilmsTime-lapse MicrocinematographySwarmδ-proteobacteriumCollective BehaviorCoordinationComplex PatternsEnvironmental CuesSelf-organizingEmergent PatternsChemotaxisNutrient GradientTime-lapse Microcinematography Tracking AssayPlate ComplexMicroscopesTime-lapse VideosQuantifiable DataAnalysis/storage ComputerM. Xanthus Community

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