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RhoC GTPase Activation Assay

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Biologie

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Journal JoVE Biologie

RhoC GTPase Activation Assay

RhoC GTPase Activation Assay

DOI:

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09:58 min

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August 22, 2010

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Michelle Lucey, Heather Unger, Kenneth L. van Golen

¹Department of Biological Sciences, The Center for Translational Cancer Research,University of Delaware

Chapitres

00:00Titre
00:48Introduction
01:25Preparation of GST-fusion Protein
04:41GST-RBD Pull Down Assay
09:01Representative Results for a RhoC GTPase Activation Assay
09:34Conclusion

Summary

Traduction automatique

This protocol utilizes a pull down assay to determine the levels of active RhoC GTPase within cells.

Tags

RhoC GTPase RhoA GTPase Activation Assay Homology Reagents Techniques Metastatic Cancer Cells Rho Activation Assay GST-Rho Binding Domain Fusion Protein Active RhoC GTPase Total Protein GTP Bound GDP Bound Commercial Versions Commercial Kits RhoC-specific Antibody

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