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A Method for Murine Islet Isolation and Subcapsular Kidney Transplantation
Journal JoVE
Médecine
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Journal JoVE Médecine
A Method for Murine Islet Isolation and Subcapsular Kidney Transplantation

A Method for Murine Islet Isolation and Subcapsular Kidney Transplantation

DOI:
10.3791/2096-v

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17:42 min

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April 13, 2011

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, ,
1Molecular and Cellular Biochemistry, Center for Molecular Neurobiology,The Ohio State University, 2Integrated Biomedical Science Graduate Program,The Ohio State University, 3Comprehensive Cancer Center,The Ohio State University

Chapitres

  • 00:05Titre
  • 01:49Cannulation and Perfusion of the Bile Duct
  • 05:07Isolation of Pancreatic Islets
  • 09:14Picking Islets
  • 12:37Islet Transplantation Procedure
  • 16:16Isolated and Transplanted Islets
  • 17:15Conclusion

Summary

Traduction automatique

Traduction automatique

Transplantation of isolated islets has been proposed to be a potential treatment for type 1 diabetes. Here we describe a method to isolate islets from mouse pancreata and transplant them to the subcapsular space of the kidney.

Tags

Islet IsolationKidney TransplantationType 1 DiabetesIslet TransplantationBlood Glucose LevelsImmunosuppressionTherapeutic UtilityMouse Kidney CapsuleSurgical StepsVideo ProtocolWritten ProtocolSyngeneic TransplantationAllogeneic Transplantation

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