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Encyclopedia of Experiments
Biological Techniques
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Encyclopedia of Experiments Biological Techniques
Nanoblade-Based Delivery of Nucleic Acid Cargo: A Technique to Deliver Cas9-sgRNA Complex to Target Cells via Virus-Like Particles

Nanoblade-Based Delivery of Nucleic Acid Cargo: A Technique to Deliver Cas9-sgRNA Complex to Target Cells via Virus-Like Particles

Transcription

For transduction of target cells with nanoblades, replace the cell culture medium with 500 microliters of the medium containing purified nanoblades. After four to six hours of cell incubation, replace the medium to the normal amount of fresh medium.

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