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腹腔注射到成年斑马鱼
Journal JoVE
Biologie
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Journal JoVE Biologie
Intraperitoneal Injection into Adult Zebrafish
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腹腔注射到成年斑马鱼

DOI:
10.3791/2126-v

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09:19 min

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August 30, 2010

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, , ,
1Department of Organismal Biology and Anatomy,The University of Chicago, 2Committee on Molecular Metabolism and Nutrition,The University of Chicago, 3Department of Medicine,The University of Chicago

Chapitres

  • 00:00Titre
  • 00:54Introduction
  • 01:19Pre-injection Preparations
  • 05:34Cold-water Anesthesia, Injection, and Recovery
  • 07:56Blood Glucose Levels After 72 Hour Fast and Glucose Injection
  • 08:41Conclusion

Summary

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Traduction automatique

我们证明到成年斑马鱼的腹腔注射。我们使用10μLNanoFil microsyringe由一个Micro4控制器和UltraMicroPump第三控制。此演示包括作为麻醉剂使用的冷水。

Tags

Intraperitoneal InjectionAdult ZebrafishReagent AbsorptionMetabolic StudiesIP InjectionAbdominal CavityPelvic GirdleVeterinary MethodsZero MortalityBleeding At Injection SiteGentle HandlingFastingWeighingAnesthetizingRecoveryStress MinimizationSmall Injection Volume35G NeedleCortland Salt SolutionOsmotically BalancedFreshwater FishAeration Of GillsSurgical Plane Of Anesthesia

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