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Generation of RNA/DNA Hybrids in Genomic DNA by Transformation using RNA-containing Oligonucleotides
Journal JoVE
Biologie
This content is Free Access.
Journal JoVE Biologie
Generation of RNA/DNA Hybrids in Genomic DNA by Transformation using RNA-containing Oligonucleotides
DOI:

16:42 min

November 24, 2010

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Chapitres

  • 00:05Titre
  • 01:20Introduction
  • 01:53Preparation of the RNA-containing Oligo and the Yeast Cells for Transformation
  • 04:40Transformation of Yeast Cells Using the RNA-Containing Oligo
  • 07:47Analysis of Gene Correction by the RNA-Containing Oligo
  • 11:10Alkali Treatment of the RNA-Containing Oligo
  • 12:20Representative Results for Transformation Using RNA-Containing Oligonucleotides
  • 16:10Conclusion

Summary

Traduction automatique

This work shows how to form an RNA/DNA hybrid at the chromosomal level and reveal transfer of genetic information from RNA to genomic DNA in yeast cells.

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