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Intracranial Orthotopic Allografting of Medulloblastoma Cells in Immunocompromised Mice
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Neurosciences
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Journal JoVE Neurosciences
Intracranial Orthotopic Allografting of Medulloblastoma Cells in Immunocompromised Mice

Intracranial Orthotopic Allografting of Medulloblastoma Cells in Immunocompromised Mice

DOI:
10.3791/2153-v

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05:10 min

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October 03, 2010

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, , , , ,
1Department of Cell and Developmental Biology,Vanderbilt University, 2Department of Neurology,Vanderbilt University

Chapitres

  • 00:00Titre
  • 00:57Introduction
  • 01:12Intracranial Grafting of Medulloblastoma Tumor Cells
  • 03:42Images of Secondary Medulloblastomas
  • 04:46Conclusion

Summary

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This protocol describes the isolation and dissociation of mouse medulloblastoma tissue, and subsequent allografting of the tumor cells into immunocompromised recipient mice in order to initiate secondary medulloblastoma.

Tags

Intracranial Orthotopic AllograftingMedulloblastoma CellsImmunocompromised MicePediatric TumorNervous SystemCerebellar Granule Neuron PrecursorsCGNPsCell-of-originClinical PresentationsMedulloblastoma SubtypesCellular And Molecular OriginsEctopic ExpressionCGNP MarkerTumor-initiating CellsExperimental ProtocolEstablished Cell Lines

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