Journal
/
Biologie
/
Adenovirus-mediated Genetic Removal of Signaling Molecules in Cultured Primary Mouse Embryonic Fibroblasts
Journal JoVE
Biologie
Author Produced
This content is Free Access.
Journal JoVE Biologie
Adenovirus-mediated Genetic Removal of Signaling Molecules in Cultured Primary Mouse Embryonic Fibroblasts

Adenovirus-mediated Genetic Removal of Signaling Molecules in Cultured Primary Mouse Embryonic Fibroblasts

DOI:
10.3791/2160-v

•

11:00 min

•

September 09, 2010

•

, ,
1Department of Molecular and Cellular Biology,University of Guelph

Chapitres

  • 00:00Titre
  • 00:27Introduction
  • 02:59Materials & Methods
  • 09:36Conclusion

Summary

Traduction automatique

Traduction automatique

In this video we use an adenovirus carrying the Cre recombinase gene to infect primary mouse embryonic fibroblasts carrying a floxed Rac1 allele.

Tags

Adenovirus-mediatedGenetic RemovalSignaling MoleculesCultured Primary Mouse Embryonic FibroblastsCre-loxP SystemConditional DeletionCre RecombinaseFloxed DNARecombination EventExcisionAdenovirusCre Recombinase GeneInfectPrimary Mouse Embryonic Fibroblasts (MEFs)Floxed Rac1 AlleleMorphological ChangesActin CytoskeletonViral TransductionCre ExpressionStained Using Phalloidin DyeConfocal Laser Scanning Microscopy

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

Live Imaging of Dense-core Vesicles in Primary Cultured Hippocampal Neurons

Generation of Induced Pluripotent Stem Cells by Reprogramming Mouse Embryonic Fibroblasts with a Four Transcription Factor, Doxycycline Inducible Lentiviral Transduction System

Dissection of Hippocampal Dentate Gyrus from Adult Mouse

Assaying the Ability of Diffusible Signaling Molecules to Reorient Embryonic Spinal Commissural Axons

In vivo Imaging and Therapeutic Treatments in an Orthotopic Mouse Model of Ovarian Cancer

Rapid Fibroblast fjerning fra High Density Menneskelig embryonale stamceller kulturer

Et system for<em> Ex vivo</em> Dyrking av Embryonic Pancreas

Rapid Genetic Analysis of epitelial-mesenchymale signaleringsprotokoll Under Hair Regeneration

Organelle Transport i Helstøpt<em> Drosophila</em> Celler: S2 Cell Line og Primær nevroner.

Glukoseopptaksmåling og respons på insulinstimulering i<em> I Vitro</em> Dyrket humant primært myotubes

Read Article