This content is Free Access.
Chapitres
- 00:05Titre
- 01:21Introduction
- 01:58Preparation of Dialyzed Cell Lysate
- 04:48Pouring of Blue Native-gels
- 07:04Separation of Dialyzed Cell Lysate by Blue Native-PAGE
- 07:53Second Dimension SDS-PAGE
- 09:25Representative Results for Blue Native-PAGE Analysis of Multiprotein Complexes from Cellular Lysates
- 11:18Conclusion
Tags
Blue Native Polyacrylamide Gel ElectrophoresisBN-PAGEMultiprotein ComplexesCell SignallingProtein-protein Interaction NetworksNative ConditionsResolutionGel FiltrationSucrose Density UltracentrifugationMPC SizeCompositionRelative AbundanceHydrodynamic Size And ShapePolyacrylamide MatrixCellular LysatesLysate DialysisFirst Dimension BN-PAGESecond Dimension SDS-PAGE
