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Atividade de testes Protozoacidal Ligand-lítica Peptídeos Contra Gut Termite Protozoa In vitro (Cultura Protozoa) e In vivo (Microinjeção em Termite intestino grosso)
Journal JoVE
Biologie
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Journal JoVE Biologie
Testing Protozoacidal Activity of Ligand-lytic Peptides Against Termite Gut Protozoa in vitro (Protozoa Culture) and in vivo (Microinjection into Termite Hindgut)
DOI:

17:36 min

December 29, 2010

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Chapitres

  • 00:05Titre
  • 01:48Introduction
  • 02:51Creation and Preparation of Peptides and Lytic Peptide Conjugates
  • 03:43Extraction of Termite Gut Protozoa Under Anaerobic Conditions
  • 06:59Binding of Fluorescent-coupled Ligand to Termite Gut Protozoa
  • 08:12Binding Ligand-coupled Lytic Peptides to Termite Gut Protozoa
  • 09:07Injecting Fluorescent-coupled Ligand into the Termite Hindgut
  • 12:08Injecting Ligand-coupled Lytic Peptide into the Termite Hindgut
  • 12:59Results: Synthesized Ligand Increases Lytic Peptide Binding and Activity Against Termite Gut Protozoa
  • 15:14Conclusion

Summary

Traduction automatique

Apresentamos os procedimentos para demonstrar que ligantes se ligam à superfície da membrana de celulose de digestão destes protozoários no intestino de cupins subterrâneos Formosan por microscopia de fluorescência e que juntamente com peptídeos ligantes líticas matar protozoários<em> In vitro</em> (Cultura protozoários anaeróbios) e<em> In vivo</em> (Injecção no intestino posterior de cupins).

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