Journal
/
Neurosciences
/
Growth and Differentiation of Adult Hippocampal Arctic Ground Squirrel Neural Stem Cells
Journal JoVE
Neurosciences
Un abonnement à JoVE est nécessaire pour voir ce contenu.  Connectez-vous ou commencez votre essai gratuit.
Journal JoVE Neurosciences
Growth and Differentiation of Adult Hippocampal Arctic Ground Squirrel Neural Stem Cells

Growth and Differentiation of Adult Hippocampal Arctic Ground Squirrel Neural Stem Cells

DOI:
10.3791/2199-v

•

09:44 min

•

January 07, 2011

•

, , ,
1Alaska Basic Neuroscience Program, Institute of Arctic Biology,University of Alaska at Fairbanks, 2Department Biochemistry,Hood College, 3Department of Cell Biology,Neuronascent, Inc., 4Research and Development,Neuronascent, Inc.

Chapitres

  • 00:05Titre
  • 01:03Thawing, Seeding, and Expanding Arctic Ground Squirrel Neural Stem Cells (agsNSC)
  • 01:05Preparing poly-L-ornithine-coated Flasks
  • 01:56Thawing and Seeding agsNSC
  • 03:39Expanding agsNSC
  • 04:11Trypsinization of Cells
  • 05:27Inoculating Well Plates for Differentiation
  • 07:25Maintenance of Neurons
  • 08:09Results of Growth and Differentiation of agsNSC
  • 09:29Conclusion

Summary

Traduction automatique

Traduction automatique

Neural stem cells were prepared from the hippocampus of adult non-hibernating yearling Arctic ground squirrels (AGS). These neural stem cells can be expanded through numerous passages, differentiated and maintained as a nearly 50:50 neuron to glial culture.

Tags

GrowthDifferentiationAdult HippocampalArctic Ground SquirrelNeural Stem CellsHibernationIschemic InjuryBrainOxygen-glucose DeprivationNeuronsProteinsGene TargetsTherapiesIschemic IndicationsCold ToleranceProliferationCultureTUJ1-positive NeuronsToxic ChemicalsNeurogenesisMEK/ERK Signaling PathwayPI3K/Akt Signaling Pathway

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

check_url/fr/2199?article_type=v&slug=growth-differentiation-adult-hippocampal-arctic-ground-squirrel

Isolation and Expansion of the Adult Mouse Neural Stem Cells Using the Neurosphere Assay
check_url/fr/2199?article_type=v&slug=growth-differentiation-adult-hippocampal-arctic-ground-squirrel

Expansion of Embryonic and Adult Neural Stem Cells by In Utero Electroporation or Viral Stereotaxic Injection
check_url/fr/2199?article_type=v&slug=growth-differentiation-adult-hippocampal-arctic-ground-squirrel

Isolation and Culture of Neural Crest Cells from Embryonic Murine Neural Tube
check_url/fr/2199?article_type=v&slug=growth-differentiation-adult-hippocampal-arctic-ground-squirrel

Promotion of Survival and Differentiation of Neural Stem Cells with Fibrin and Growth Factor Cocktails after Severe Spinal Cord Injury
check_url/fr/2199?article_type=v&slug=growth-differentiation-adult-hippocampal-arctic-ground-squirrel

Growing Neural Stem Cells from Conventional and Nonconventional Regions of the Adult Rodent Brain
check_url/fr/2199?article_type=v&slug=growth-differentiation-adult-hippocampal-arctic-ground-squirrel

One Mouse, Two Cultures: Isolation and Culture of Adult Neural Stem Cells from the Two Neurogenic Zones of Individual Mice
check_url/fr/2199?article_type=v&slug=growth-differentiation-adult-hippocampal-arctic-ground-squirrel

Enumeration of Neural Stem Cells Using Clonal Assays
check_url/fr/2199?article_type=v&slug=growth-differentiation-adult-hippocampal-arctic-ground-squirrel

Isolation and Culture of Adult Neural Stem Cells from the Mouse Subcallosal Zone
check_url/fr/2199?article_type=v&slug=growth-differentiation-adult-hippocampal-arctic-ground-squirrel

Development of a Refined Protocol for Trans-scleral Subretinal Transplantation of Human Retinal Pigment Epithelial Cells into Rat Eyes
check_url/fr/2199?article_type=v&slug=growth-differentiation-adult-hippocampal-arctic-ground-squirrel

Isolation and Culture of Embryonic Mouse Neural Stem Cells

Read Article