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JoVE Journal Neuroscience
Growth and Differentiation of Adult Hippocampal Arctic Ground Squirrel Neural Stem Cells
 
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Growth and Differentiation of Adult Hippocampal Arctic Ground Squirrel Neural Stem Cells

Article DOI: 10.3791/2199-v • 09:44 min • January 7th, 2011
January 7th, 2011 •
1, 2, 3, 4
1Alaska Basic Neuroscience Program, Institute of Arctic Biology, University of Alaska at Fairbanks, 2Department Biochemistry, Hood College, 3Department of Cell Biology, Neuronascent, Inc., 4Research and Development, Neuronascent, Inc.

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Neural stem cells were prepared from the hippocampus of adult non-hibernating yearling Arctic ground squirrels (AGS). These neural stem cells can be expanded through numerous passages, differentiated and maintained as a nearly 50:50 neuron to glial culture.

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Growth Differentiation Adult Hippocampal Arctic Ground Squirrel Neural Stem Cells Hibernation Ischemic Injury Brain Oxygen-glucose Deprivation Neurons Proteins Gene Targets Therapies Ischemic Indications Cold Tolerance Proliferation Culture TUJ1-positive Neurons Toxic Chemicals Neurogenesis MEK/ERK Signaling Pathway PI3K/Akt Signaling Pathway
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