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Chapitres
- 00:01Introducton
- 00:23Critical Reagents
- 01:05Propagate the Virus in C6/36 Cell Line
- 02:49Prepare the Mixture of Virus and Blood
- 04:29Feeding Mosquitoes
- 07:25Assay of Virus Titer in Mosquito Tissue
- 11:435 Day Incubation
- 14:10Conclusion
- 15:13End Credits
Once a gene is identified as potentially refractory for the dengue virus, it must be evaluated for it's role in preventing viral infections within the mosquito. This protocol illustrates how the extent of dengue infections of mosquitoes can be assayed. The techniques for growing up the virus in culture, membrane feeding mosquitoes human blood, and assaying viral titers in the mosquito midgut are demonstrated.
