Preparation of Highly Coupled Rat Heart Mitochondria

Journal

Biologie

Journal JoVE
Biologie
Author Produced

This content is Free Access.

Journal JoVE Biologie

Preparation of Highly Coupled Rat Heart Mitochondria

DOI:

10.3791/2202-v

•

12:28 min

•

September 23, 2010

•

Irina Gostimskaya, Alexander Galkin

¹Faculty of Life Sciences,University of Manchester, ²School of Biological Sciences,Queen’s University Belfast

Chapitres

00:00Titre
00:07Introduction
00:14General Scheme of the Protocol
12:19Conclusion

Summary

Traduction automatique

We describe а protocol for isolation of pure, highly coupled rat heart mitochondria for functional or structural studies of cellular bioenergetics, biophysical measurements, proteomics or mitochondrial DNA and lipids analysis.

Preparation of Highly Coupled Rat Heart Mitochondria

•

•

•

Chapitres

Summary

Traduction automatique

Tags

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

Implantation of Engineered Tissue in the Rat Heart

Primary Culture of Adult Rat Heart Myocytes

Purification of Mitochondria from Yeast Cells

Preparation of embryos for Electron Microscopy of the Drosophila embryonic heart tube

Preparation of Rat Tail Tendons for Biomechanical and Mechanobiological Studies

Multi-parameter Measurement of the Permeability Transition Pore Opening in Isolated Mouse Heart Mitochondria

Confocal Imaging of Single Mitochondrial Superoxide Flashes in Intact Heart or In Vivo

Quantifying Single Microvessel Permeability in Isolated Blood-perfused Rat Lung Preparation

Preparation of Rat Serum Suitable for Mammalian Whole Embryo Culture

Automated Modular High Throughput Exopolysaccharide Screening Platform Coupled with Highly Sensitive Carbohydrate Fingerprint Analysis

Read Article