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Microfabricated Platforms for Mechanically Dynamic Cell Culture
Journal JoVE
Bio-ingénierie
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Journal JoVE Bio-ingénierie
Microfabricated Platforms for Mechanically Dynamic Cell Culture
DOI:

15:21 min

December 26, 2010

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Chapitres

  • 00:05Titre
  • 01:05Introduction
  • 01:31Sandwich Mold Fabrication
  • 03:27Constructing the Multilayer Device
  • 06:45Microactuator Modification for Active 2D Substrates
  • 10:01Device Modifications for Active 3D Hydrogels
  • 12:11In Situ Polymerization
  • 13:51Representative Results for Device Fabrication, Characterization, and Operation
  • 14:57Conclusion

Summary

Traduction automatique

In this protocol, we demonstrate the fabrication of a microactuator array of vertically displaced posts on which the technology is based, and how this base technology can be modified to conduct high-throughput mechanically dynamic cell culture in both two-dimensional and three-dimensional culture paradigms.

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