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Chapitres
- 00:02Introduction
- 01:24Preparing Spinal Cord Emulsion
- 08:43Loading Glass Syringes with Emulsion
- 12:37Injecting Rats with Spinal Cord Emulsion
- 15:51Monitoring Clinical Signs (CS)
- 17:31CS 0.0 – No Symptoms of EAE
- 18:50CS 0.5 – Distal Limp Tail
- 19:29CS 1.0 – Limp Tail
- 20:21CS 2.0 – Weakened Hindlimbs
- 21:59CS 3.0 – Moderate Hindlimb Paralysis
- 23:06CS 3.5 – Paralysis of Single Hindlimb
- 23:28CS 4.0 – Complete Hindlimb Paralysis
- 23:44CS 5.0 – Complete Hindlimb Paralysis and Incontinence
- 24:07Caring for Animals with EAE
- 24:53Conclusion
- 26:39End Credits
Questo video dimostra il punteggio di induzione e clinica di un modello animale di sclerosi multipla: cronico-recidivante encefalomielite autoimmune sperimentale nei ratti DA. La malattia, indotta da immunizzando topi con una emulsione che contiene tutto midollo spinale di ratto e adiuvante completo di Freund, presenta segni clinici simile alla malattia umana.
Tags
InductionClinical ScoringChronic-Relapsing Experimental Autoimmune EncephalomyelitisMultiple SclerosisChronic Inflammatory DiseaseCentral Nervous SystemDemyelinationGlial ScaringBrainSpinal CordDemyelinated PlaquesParaparesisParalysisBlindnessIncontinenceExperimental Autoimmune Encephalomyelitis (EAE)MS ModelVaccinationRabiesViruses Grown On Rabbit Spinal CordsCNS DiseaseNon-human Primates Immunized With A CNS HomogenateSpecies/strainImmunizing AntigenLewis RatsMyelin Basic Protein In Emulsion With AdjuvantGuinea PigsDA RatsDA Rat Spinal CordComplete Freund’s AdjuvantAscending Flaccid Paralysis
