Journal
/
Immunologie et infection
/
A 96 Well Microtiter Plate-based Method for Monitoring Formation and Antifungal Susceptibility Testing of Candida albicans Biofilms
Journal JoVE
Immunologie et infection
Un abonnement à JoVE est nécessaire pour voir ce contenu.  Connectez-vous ou commencez votre essai gratuit.
Journal JoVE Immunologie et infection
A 96 Well Microtiter Plate-based Method for Monitoring Formation and Antifungal Susceptibility Testing of Candida albicans Biofilms

A 96 Well Microtiter Plate-based Method for Monitoring Formation and Antifungal Susceptibility Testing of Candida albicans Biofilms

DOI:
10.3791/2287-v

•

07:19 min

•

October 21, 2010

•

, , ,
1Department of Biology,University of Texas San Antonio – UTSA, 2South Texas Center for Emerging Infectious Diseases,University of Texas San Antonio – UTSA

Chapitres

  • 00:00Titre
  • 01:24Preparation of C. albicans
  • 02:49Setting Up the 96-well Microtiter Plate for the Formation of the Biofilm
  • 03:55Antifungal Susceptibility Testing of Biofilms
  • 06:02Evaluation of Antifungal Reagents
  • 06:43Conclusion

Summary

Traduction automatique

Traduction automatique

We describe a simple, rapid and robust method for the formation of Candida albicans biofilms using 96 well microtiter plates and its utility in antifungal susceptibility testing of cells within biofilms.

Tags

96 Well Microtiter PlateMonitoringFormationAntifungal Susceptibility TestingCandida AlbicansBiofilmsCandidiasisFungal InfectionsMedical DevicesCathetersIn Vitro ModelColorimetric AssayXTTTetrazolium SaltMetabolically Active Fungal Biofilm CellsExperiment DurationAntifungal Susceptibility Testing Standardization

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

check_url/fr/2287?article_type=v

Microtiter Dish Biofilm Formation Assay
check_url/fr/2287?article_type=v

In vitro Biofilm Formation in an 8-well Chamber Slide
check_url/fr/2287?article_type=v

Antimicrobial Susceptibility Testing of Mycobacterium Tuberculosis Complex for First and Second Line Drugs by Broth Dilution in a Microtiter Plate Format
check_url/fr/2287?article_type=v

Preparation of a Blood Culture Pellet for Rapid Bacterial Identification and Antibiotic Susceptibility Testing
check_url/fr/2287?article_type=v

A Reference Broth Microdilution Method for Dalbavancin In Vitro Susceptibility Testing of Bacteria that Grow Aerobically
check_url/fr/2287?article_type=v

Visualization of Biofilm Formation in Candida albicans Using an Automated Microfluidic Device
check_url/fr/2287?article_type=v

Deep Dermal Injection As a Model of Candida albicans Skin Infection for Histological Analyses
check_url/fr/2287?article_type=v

Daily Phototherapy with Red Light to Regulate Candida albicans Biofilm Growth
check_url/fr/2287?article_type=v

Clarifying and Imaging Candida albicans Biofilms
check_url/fr/2287?article_type=v

Rose Bengal-Mediated Photodynamic Therapy to Inhibit Candida albicans

Read Article