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Analysis of Pluripotent Stem Cells by using Cryosections of Embryoid Bodies
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Biologie
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Journal JoVE Biologie
Analysis of Pluripotent Stem Cells by using Cryosections of Embryoid Bodies

Analysis of Pluripotent Stem Cells by using Cryosections of Embryoid Bodies

DOI:
10.3791/2344-v

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06:54 min

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December 08, 2010

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, , , ,
1Laboratório Nacional de Céulas-Tronco Embrionárias (LaNCE), Instituto de Ciências Biomédicas,Universidade Federal do Rio de Janeiro (UFRJ), Brazil

Chapitres

  • 00:02Titre
  • 00:07Introduction
  • 00:37Materials and Equipment
  • 01:09Preparing Embryoid Body Cryo-Blocks
  • 05:14Cutting
  • 06:37Conclusion

Summary

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Pluripotent stem cells growing in suspension differentiate into embryoid bodies (EBs). Here we demonstrate how to obtain high quality EB cryosections useful for studying cellular and molecular aspects of embryogenesis, while preserving their organization as aggregates.

Tags

Pluripotent Stem CellsCryosectionsEmbryoid BodiesES CellsDifferentiationAggregatesEctodermMesodermEndodermImmunofluorescenceIn Situ HybridizationTarget ProteinsMRNACryosection TechniqueAnalytical ProceduresHistology Staining AnalysisHematoxilin And EosinDAPIOct4NestinEmbryogenesisTri-dimensional Spherical Structure

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