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Whole-Cell Recording of Calcium Release-Activated Calcium (CRAC) Currents in Human T Lymphocytes
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Journal JoVE Biologie
Whole-Cell Recording of Calcium Release-Activated Calcium (CRAC) Currents in Human T Lymphocytes

Whole-Cell Recording of Calcium Release-Activated Calcium (CRAC) Currents in Human T Lymphocytes

DOI:
10.3791/2346-v

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15:29 min

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December 21, 2010

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1Department of Physiology and Membrance Biology,University of California, Davis

Chapitres

  • 00:05Titre
  • 02:04Experimental Preparation
  • 04:15Mounting Cells on the Microscope Stage
  • 05:25CRAC Channel Current Recording
  • 11:43Data Analysis
  • 12:20Representative CRAC Channels Recorded in a Resting Human T Lymphocyte
  • 14:39Conclusion

Summary

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We provide a step-by-step protocol for whole-cell patch clamp recording of Calcium Release-Activated Calcium (CRAC) currents in peripheral blood mononuclear cell-derived human T lymphocytes.

Tags

Whole-cell RecordingCalcium Release-activated Calcium CurrentsCRAC ChannelsT LymphocytesIntracellular Ca2+ StorePlasmalemmal Ca2+ ChannelsT Cell ProliferationGene ExpressionSevere Combined ImmunodeficiencyAutoimmune DiseasesFunctional Channel PropertiesRegulationJurkat T CellsHuman Leukemia T Cell LineNormal Human T CellsBlood-derived T LymphocytesAmplitudeCa2+ CurrentsNa+ Currents

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