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Paired Patch Clamp Recordings from Motor-neuron and Target Skeletal Muscle in Zebrafish
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Chapitres
- 00:00Titre
- 01:21Preparation of Fish for Paired Recordings
- 03:35Paired Recordings of CaP Motor Neuron and Target Muscle Cells
- 06:41Conclusion
Larval zebrafish represent the first vertebrate model system to allow simultaneous patch clamp recording from a spinal motor-neuron and target skeletal muscle. This video demonstrates the microscopic methods used to identify a segmental CaP motor-neuron and target muscle cells as well as the methodologies for recording from each cell type.
Tags
Patch Clamp RecordingMotor-neuronTarget MuscleZebrafish LarvaVertebrate Model SystemCaP Motor-neuronMorphologyLocationMicroscopic MethodsDye FillingAction Potential WaveformCell Input ResistanceLong-term RecordingsFrequency-dependence Of Synaptic TransmissionNeuromuscular JunctionQuantal SizeWhole Cell Voltage ClampUnitary EventsRelease PropertiesVesicle RecyclingSynaptic DepressionSynaptic FacilitationIn Vivo Preparation
