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Utero Intraventricular और E16 चूहा भ्रूण के इंजेक्शन electroporation में
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JoVE Journal
Biology
In Utero Intraventricular Injection and Electroporation of E16 Rat Embryos
Please note that all translations are automatically generated.
Click here for the English version.
Utero Intraventricular और E16 चूहा भ्रूण के इंजेक्शन electroporation में
DOI:
10.3791/236-v
•
19:04 min
•
July 19, 2007
•
William Walantus
,
Laura Elias
,
Arnold Kriegstein
1
Institute for Regeneration Medicine
,
University of California, San Francisco – UCSF
Chapters
00:00
Title
00:17
Introduction
01:06
Tools and Reagents
02:17
Preparation of Microinjection Pipets
06:23
Prepping Rat for Surgery
10:02
Removing Uterus and Exposing Embryos
12:12
In utero Injection and Electroporation
14:11
Placing Embryos Back Inside Dam
17:23
Conclusion
18:48
End Credits
Tags
In-utero Intraventricular Injection
Electroporation
E16 Rat Embryos
Neocortex Manipulation
Progenitor Cells
Lateral Ventricle
Corticogenesis Study
Gene Over-expression
Gene Knockdown
Cellular Proliferation
Migration
Differentiation
Knockout Strategies
Temporal Window
Surgery Preparation
Laporatomy
DNA Injection
Progenitor Cells Lining The Lateral Wall
Post-surgery Care
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