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Ex Vivo Culture of Patient Tissue & Examination of Gene Delivery
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Médecine
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Journal JoVE Médecine
Ex Vivo Culture of Patient Tissue & Examination of Gene Delivery

Ex Vivo Culture of Patient Tissue & Examination of Gene Delivery

DOI:
10.3791/2378-v

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05:00 min

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December 20, 2010

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, , , , , ,
1Cork Cancer Research Centre, Mercy University Hospital and Leslie C. Quick Jnr. Laboratory,University College Cork, 2Department of Computer Science,University College Cork

Chapitres

  • 00:01Titre
  • 01:16Materials
  • 01:58Slicing
  • 03:17Gene Delivery
  • 04:37Luciferase Imaging

Summary

Traduction automatique

Traduction automatique

This article describes the culture of patient tissue slices for gene delivery studies and subsequent analysis of gene expression using IVIS bioluminescence imaging.

Tags

Ex Vivo CultureGene DeliveryGene ExpressionBioluminescence ImagingIVIS Detection SystemGene QuantificationHeterogeneous NatureIndividual PatientsAnimal ModelsPreclinical ModeClinical Trial

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