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生成高度丰富的神经文化皮层组织块冷冻保存

Journal JoVE
Neurosciences
Author Produced

This content is Free Access.

Journal JoVE Neurosciences

Cryopreservation of Cortical Tissue Blocks for the Generation of Highly Enriched Neuronal Cultures

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生成高度丰富的神经文化皮层组织块冷冻保存

DOI:

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09:13 min

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November 11, 2010

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Ardeshir S. Rahman, Shaudee Parvinjah, Michael A. Hanna, Pablo R. Helguera, Jorge Busciglio

¹Department of Neurobiology and Behavior,University of California, Irvine

Chapitres

00:00Titre
01:15Material Preparation
02:41Tissue Preparation
04:20Cryopreservation
06:05Thaw and Culture
08:42Conclusion

Summary

Traduction automatique

在这里，我们描述了一个高效的冷冻保存的方法和解冻皮质的脑组织块生成神经元文化的高度浓缩。这个简单的协议提供了神经元，星形胶质细胞和神经元前体细胞培养后一代的灵活性。

Tags

Cryopreservation Neuronal Cultures Freezing Medium Dimethyl Sulfoxide (DMSO)Hank’s Buffered Salt Solution (HBSS)Cryovials Rate-controlled Freezing Container Post-thaw Processing Dissociation Neuronal-enriched Cultures Neuritic Growth Immunocytochemical Staining Astrocytic Marker Glial Fibrillary Acidic Protein (GFAP)Neuronal Marker Beta-tubulin Class III Neural Precursor Cell Cultures Neurospheres Differentiating Conditions

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