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Analysis of mRNA Nuclear Export Kinetics in Mammalian Cells by Microinjection
Journal JoVE
Biologie
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Journal JoVE Biologie
Analysis of mRNA Nuclear Export Kinetics in Mammalian Cells by Microinjection

Analysis of mRNA Nuclear Export Kinetics in Mammalian Cells by Microinjection

DOI:
10.3791/2387-v

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11:32 min

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December 04, 2010

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, , , ,
1Department of Biochemistry,University of Toronto

Chapitres

  • 00:05Titre
  • 01:23Preparation for Microinjection
  • 02:41Intranuclear Microinjection
  • 05:46Cell Fixation and Staining
  • 08:48Representative Results for mRNA Nuclear Export Kinetics
  • 10:23Conclusion

Summary

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Here we describe an assay that employs the power of microinjection coupled with fluorescent in situ hybridization in order to accurately measure the nuclear export kinetics of mRNA in mammalian somatic cells.

Tags

MRNA Nuclear Export KineticsMammalian CellsMicroinjectionEukaryotesCytoplasmTranslation MachineryXenopus OocytesYeastDrosophila Derived S2 Cell LineKinetic DataFluorescent In Situ Hybridization (FISH)Splicing Dependent MannerSignal Sequence Coding Region (SSCR)In Vitro Synthesized MRNAPlasmid DNASub-cellular Localization

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