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Respirometric Oxidative Phosphorylation Assessment in Saponin-permeabilized Cardiac Fibers
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Journal JoVE Biologie
Respirometric Oxidative Phosphorylation Assessment in Saponin-permeabilized Cardiac Fibers

Respirometric Oxidative Phosphorylation Assessment in Saponin-permeabilized Cardiac Fibers

DOI:
10.3791/2431-v

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11:10 min

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February 28, 2011

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, , ,
1Department of Biochemistry and Molecular Biology, Faculty of Medicine,University of Calgary, 2Faculty of Kinesiology,University of Calgary

Chapitres

  • 00:05Titre
  • 01:16Cardiac Fibers Mechanical Preparation
  • 02:48Cardiac Fiber Bundle Chemical Preparation and Washing
  • 03:58Wet Weight Determination
  • 04:39Respirometric OXPHOS Using Substrate-Inhibitor-Uncoupler Titrations
  • 08:33Representative Results for Respirometric OXPHOS
  • 10:43Conclusion

Summary

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Traduction automatique

Saponin-permeabilized fiber preparation in conjunction with respirometric oxidative phosphorylation analysis provides integrative assessment of mitochondrial function. Mitochondrial respiration in physiological and pathological states can reflect various regulatory influences including mitochondrial interactions, morphology and biochemistry.

Tags

Respirometric Oxidative PhosphorylationSaponin-permeabilized Cardiac FibersMitochondrial FunctionEnergy MetabolismOxidative StressApoptosisAgingMitochondrial EncephalomyopathiesDrug ToxicityClark ElectrodeSubstrate-inhibitor-uncoupler Titration ProtocolsElectron Transport ChainSarcolemmaMuscle FibersSaponin-skinned Cardiac FibersOxygen Consumption Measurements

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