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Neuroscience

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JoVE Journal Neuroscience
Slice Preparation, Organotypic Tissue Culturing and Luciferase Recording of Clock Gene Activity in the Suprachiasmatic Nucleus
 

Slice Preparation, Organotypic Tissue Culturing and Luciferase Recording of Clock Gene Activity in the Suprachiasmatic Nucleus

Article DOI: 10.3791/2439-v • 10:07 min • February 15th, 2011
February 15th, 2011 •
*1, *1, 1, 1
1Swedish Medical Nanoscience Center, Department of Neuroscience, Karolinska Institutet
* These authors contributed equally

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résumé
Automatic Translation

The procedure of preparing slices containing the adult mouse hypothalamic suprachiasmatic nucleus (SCN), and a rapid way to culture the SCN tissue in organotypic culture condition, are reported. Further, the measurement of oscillatory clock gene protein expression using dynamic luciferase reporter technology is described.

Tags

Slice Preparation Organotypic Tissue Culturing Luciferase Recording Clock Gene Activity Suprachiasmatic Nucleus Circadian Clock Physiology Behavior Retina Optic Nerve Negative Feedback Loops Clock Genes Period Genes Core Clock Membrane Depolarization Calcium CAMP Oscillations Gene Expression Metabolic Activity Electrical Activity In Vitro Study Molecular Investigations Electrophysiological Investigations Metabolic Investigations
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