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Chapitres
- 00:05Titre
- 02:01Preparation of Cell Line with an Integrated Recombination Substrate
- 03:04Transfection to Deplete Endogenous BRCA1 and add back Mutant BRCA1
- 05:19Analysis of Transfectants
- 06:07Analysis of the Effects of BRCA1 Mutations on Homologous Recombination via GFP-Expression
- 07:54Conclusion
Tags
BRCA1 MutationsHomologous RecombinationEndogenous BRCA1Missense MutationsFunctional AnalysisCell LinesRNAi TargetingBRCA1 MRNABRCA1 VariantAcute SilencingSecondary MutationsCompensation For Loss Of FunctionHeLa-derived Cell LineHomologous Recombination AssayRecombination SubstrateI-SceI Restriction Enzyme SiteGFP Allele
