JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Biology
/
Purifying Plasmid DNA from Bacterial Colonies Using the Qiagen Miniprep Kit
JoVE Journal
Biology
A subscription to JoVE is required to view this content.
Sign in or start your free trial.
JoVE Journal
Biology
Purifying Plasmid DNA from Bacterial Colonies Using the Qiagen Miniprep Kit
Purifying Plasmid DNA from Bacterial Colonies Using the Qiagen Miniprep Kit
DOI:
10.3791/247-v
•
09:24 min
•
July 29, 2007
•
Shenyuan Zhang
,
Michael D. Cahalan
1
Department of Physiology and Biophysics
,
University of California, Irvine (UCI)
Chapters
00:07
Introduction
00:46
Growing Up Bacteria
01:24
Resuspending and Lysing Bacteria with Buffers P1 and P2
03:56
Precipitating Bacterial Protein with Buffer N3
04:38
Assembling the Qiavac Manifold during Centrifugation
05:15
Binding Plasmid DNA to Qiastrip and Washing with Buffers PB and PE
07:19
Eluting DNA in Collection Tubes
08:48
Conclusion
Tags
Qiagen Miniprep Kit
Plasmid DNA Purification
Bacterial Colonies
E. Coli
Clone Verification
DNA Isolation
Enzymatic Reactions
Polymerase Chain Reaction
Restriction Enzyme Digestion
Alkaline Lysis
High Salt Adsorption
Silica Membrane
Washing
Elution
Phenol-free Purification
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
check_url/247?article_type=v
Transformation of Plasmid DNA into E. coli Using the Heat Shock Method
check_url/247?article_type=v
Immunohistochemistry: Paraffin Sections Using the Vectastain ABC Kit from Vector Labs
check_url/247?article_type=v
Enrichment of NK Cells from Human Blood with the RosetteSep Kit from StemCell Technologies
check_url/247?article_type=v
RNA Extraction from Neuroprecursor Cells Using the Bio-Rad Total RNA Kit
check_url/247?article_type=v
Streamlined Purification of Plasmid DNA From Prokaryotic Cultures
check_url/247?article_type=v
Velge og isolere kolonier av menneskeskapte pluripotent stamceller omprogrammert fra voksne Fibroblaster
check_url/247?article_type=v
Mus Sperm Kryokonservering og Recovery bruke I · Cryo Kit
check_url/247?article_type=v
Rense Impure: Sekvense Metagenomes og Metatranscriptomes fra Komplekse Animal-assosiert Samples
check_url/247?article_type=v
En hybrid DNA Extraction Method for kvalitativ og kvantitativ vurdering av bakteriesamfunn fra fjærfe produksjon Samples
check_url/247?article_type=v
Plasmid-avledet DNA Strand Displacement Gates for Implementing kjemisk reaksjon Networks
Read Article