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Purification of Progenitors from Skeletal Muscle
Journal JoVE
Biologie
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Journal JoVE Biologie
Purification of Progenitors from Skeletal Muscle

Purification of Progenitors from Skeletal Muscle

DOI:
10.3791/2476-v

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12:55 min

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March 16, 2011

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,
1The Biomedical Research Centre,University of British Columbia

Chapitres

  • 00:05Titre
  • 02:35Tissue Collection
  • 03:49Dissociation of Tissue into Single Cells by Enzymatic Digestion
  • 05:52Antibody Staining and Sorting
  • 08:45Transplantation
  • 09:45Representative Myogenic and Adipogenic Progenitor Isolation and Transplantation Results
  • 11:05Conclusion

Summary

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Traduction automatique

Method for the enzymatic dissociation, surface labeling and purification by flow cytometry of fibro/adipogenic and myogenic progenitors from murine skeletal muscle.

Tags

PurificationProgenitorsSkeletal MuscleMyogenic ProgenitorsSatellite CellsBasement MembraneVessel-associated ProgenitorsMyofibroblastsWhite AdipocytesProliferationTrophic SupportAssaysIsolationTransplantationFlow CytometryEnzymatic DissociationColony-forming CellsCell TypesMolecular Analysis

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