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纯化的骨骼肌的先觉者

Journal JoVE
Biologie

This content is Free Access.

Journal JoVE Biologie

Purification of Progenitors from Skeletal Muscle

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纯化的骨骼肌的先觉者

DOI:

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12:55 min

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March 16, 2011

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Lin Yi, Fabio Rossi

¹The Biomedical Research Centre,University of British Columbia

Chapitres

00:05Titre
02:35Tissue Collection
03:49Dissociation of Tissue into Single Cells by Enzymatic Digestion
05:52Antibody Staining and Sorting
08:45Transplantation
09:45Representative Myogenic and Adipogenic Progenitor Isolation and Transplantation Results
11:05Conclusion

Summary

Traduction automatique

酶解，表面标记和流式细胞仪检测纤维/成脂和小鼠骨骼肌生肌祖净化的方法。

Tags

Purification Progenitors Skeletal Muscle Myogenic Progenitors Satellite Cells Basement Membrane Vessel-associated Progenitors Myofibroblasts White Adipocytes Proliferation Trophic Support Assays Isolation Transplantation Flow Cytometry Enzymatic Dissociation Colony-forming Cells Cell Types Molecular Analysis

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