Whole Cell Recordings from Brain of Adult Drosophila

Huaiyu Gu; Diane K. O'Dowd

doi:10.3791/248

Journal

/

Biologie

/

Todo Recordings celular do cérebro de adultos Drosophila

Journal JoVE
Biologie

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Journal JoVE Biologie

Whole Cell Recordings from Brain of Adult Drosophila

Todo Recordings celular do cérebro de adultos Drosophila

DOI:

10.3791/248-v

•

09:27 min

•

July 29, 2007

•

Huaiyu Gu, Diane K. O’Dowd

¹Department of Development and Cell Biology, Department of Anatomy and Neurobiology,University of California, Irvine (UCI)

Chapitres

00:04Titre
00:22Introduction
00:53Preparation of Dissection Solution
02:15Adult Drosophila Selection
02:32Sex Differentiation
03:04Decapitation and Removal of Brain
04:19Removal of Trachea and Enzyme Treatment
05:27Stabilizing Brain in Recording Chamber
07:58Conclusion

Summary

Traduction automatique

Este vídeo demonstra o procedimento para isolar toda cérebros de adultos Drosophila em preparação para gravar a partir de neurônios individuais usando a tecnologia de célula padrão todo. Inclui imagens de células GFP rotulados e neurônios visto durante a gravação.