Whole Cell Recordings from Brain of Adult Drosophila

Huaiyu Gu; Diane K. O'Dowd

doi:10.3791/248

Journal

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Biologie

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Whole Cell Recordings from Brain of Adult Drosophila

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Biologie

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Journal JoVE Biologie

Whole Cell Recordings from Brain of Adult Drosophila

DOI:

10.3791/248-v

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09:27 min

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July 29, 2007

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Huaiyu Gu, Diane K. O’Dowd

¹Department of Development and Cell Biology, Department of Anatomy and Neurobiology,University of California, Irvine (UCI)

Chapitres

00:04Titre
00:22Introduction
00:53Preparation of Dissection Solution
02:15Adult Drosophila Selection
02:32Sex Differentiation
03:04Decapitation and Removal of Brain
04:19Removal of Trachea and Enzyme Treatment
05:27Stabilizing Brain in Recording Chamber
07:58Conclusion

Summary

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This video demonstrates the procedure for isolating whole brains from adult Drosophila in preparation for recording from single neurons using standard whole cell technology. It includes images of GFP labeled cells and neurons viewed during recording.

Whole Cell Recordings from Brain of Adult Drosophila

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Chapitres

Summary

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