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Chapitres
- 00:05Titre
- 01:24Introduction
- 02:09Protein Reconstitution in LCP (Lipid Cubic Phase)
- 03:42Lipid Cubic Phase – Fluorescence Recovery After Photobleaching (LCP-FRAP) Pre-Crystallization Assay
- 06:55Preparation and Harvest of LCP Crystals
- 09:25Representative Results: Crystallization of Human Beta 2 Adrenergic G Protein-Coupled Receptor
- 11:06Conclusion
Protokolleri LCP kristalizasyon denemeler ve hasat kristalleri kurma, LCP-sıkı bağlamak kristalleşme öncesi testlerin başlangıç koşulları bulma lipidik kübik bir fazlı (LCP) protein, sulandırıldıktan itibaren bir membran proteini kırınım kaliteli kristaller elde etmek için gerekli adımları açıklar .
Tags
CrystallizationMembrane ProteinsLipidic MesophasesSignal TransductionTransportEnergy TransformationsMalfunctionsDiseasesStructural UnderstandingFunctional UnderstandingSolubilizationDiffraction Quality CrystalsIn Meso CrystallizationNative-like Membrane EnvironmentSolvent ContentOrderingTraditional CrystallizationDetergent SolutionsLipid Phase BehaviorViscous Mesophase MaterialsLCP (Lipid Cubic Phase)Syringe MixerReconstitutionLipid BilayerAssay PlateCrystallization Plate
