Un abonnement à JoVE est nécessaire pour voir ce contenu. Connectez-vous ou commencez votre essai gratuit.
Chapitres
- 00:05Titre
- 00:45Introduction
- 02:11Making the Embryo Collection Cups
- 02:31Adding GFP Flies to Embryo Collection Cups
- 04:43Preparing a Mounting Chamber and Mounting Embryos
- 07:15Imaging Embryos
- 07:55Alternative Method: Mounting Embryos with Embryo -glue”
- 09:30Results: Time-lapse Imaging of Cellularization
- 10:34Conclusion
Early development of the fruit fly, Drosophila melanogaster, is characterized by a number of cell shape changes that are well suited for imaging approaches. This article will describe basic tools and methods required for live confocal imaging of Drosophila embryos, and will focus on a cell shape change called cellularization.
