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小鼠呼吸道上皮细胞和实验香烟烟雾暴露在空气分离液界面

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Médecine

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Journal JoVE Médecine

Isolation of Mouse Respiratory Epithelial Cells and Exposure to Experimental Cigarette Smoke at Air Liquid Interface

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小鼠呼吸道上皮细胞和实验香烟烟雾暴露在空气分离液界面

DOI:

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10:47 min

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February 21, 2011

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Hilaire C. Lam², Augustine M.K. Choi, Stefan W. Ryter

¹Department of Medicine, Pulmonary and Critical Care Medicine, Brigham and Women’s Hospital,Harvard Medical School, ²Cellular and Molecular Pathology, School of Medicine,University of Pittsburgh

Chapitres

00:00Titre
01:15Dissection of Mouse Tracheas
03:40Isolation of Mouse Tracheobronchial Epithelial Cells (MTEC)
08:25Application of Cigarette Smoke to Cultured Epithelial Cells
09:44Representative Results of Healthy Mouse Respiratory Epithelial Cell Cultures
10:28Conclusion

Summary

Traduction automatique

从小鼠的呼吸道和肺上皮细胞可以分离，气液界面的差异化的呼吸道上皮模式培养。协议是分离，培养，这些细胞暴露卷烟主流烟雾，为了研究这种环境毒素的分子反应。

Tags

Mouse Respiratory Epithelial Cells Isolation Experimental Cigarette Smoke Air-liquid Interface (ALI)Differentiated Respiratory Epithelium Mainstream Cigarette Smoke (CS) Exposure Airway Epithelial Cells Trachea Fully Differentiated Epithelial Cells Calibrated Mainstream CS Delivery ALI Culture System Respiratory Epithelia Physiological Setting Molecular Responses Lung Cellular Responses Environmental Air Pollution Human Health Chronic Obstructive Pulmonary Disease (COPD)Tobacco-related Diseases

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