Un abonnement à JoVE est nécessaire pour voir ce contenu. Connectez-vous ou commencez votre essai gratuit.
Chapitres
- 00:05Titre
- 01:30Pulmonary Infection of Mice by Intratracheal Intubation
- 03:32Preparation of Mice for Bioluminescence Imaging
- 04:24Bioluminescence Imaging
- 05:56Ex vivo Imaging and CFU Analysis for the Quantification of Bacteria in the Lungs
- 07:07Analysis of Luminescence Imaging
- 08:53Luminescent Imaging of Mice Intra-tracheally Infected with CBRluc-tagged Bacteria
- 09:58Conclusion
Methods for bioluminescence imaging of bacterial infections in living animals are decribed. Pathogens are modified to express luciferase allowing optical whole body imaging of infections in live animals. Animal models can be infected with luciferase expressing pathogens and the resulting course of disease visualized in real-time by bioluminescence imaging.
Tags
LuciferaseImagingBacterial InfectionsMiceBioluminescence ImagingReal-time MonitoringCancer CellsStem CellsImmune Cell TypesViral PathogensParasitesConventional StrategiesAnimal ModelsOptical ImagingInoculationVariability Between AnimalsReduce Animal NumbersData CollectionRecombinant Strains Of Bacteria Expressing LuciferaseClick Beetle LuciferaseFirefly Luciferase
