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Optical Imaging of Neurons in the Crab Stomatogastric Ganglion with Voltage-sensitive Dyes
Journal JoVE
Neurosciences
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Journal JoVE Neurosciences
Optical Imaging of Neurons in the Crab Stomatogastric Ganglion with Voltage-sensitive Dyes

Optical Imaging of Neurons in the Crab Stomatogastric Ganglion with Voltage-sensitive Dyes

DOI:
10.3791/2567-v

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09:36 min

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March 23, 2011

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, ,
1Institute of Neurobiology,Ulm University, 2School of Computing Science & Institute of Neuroscience,Newcastle University

Chapitres

  • 00:05Titre
  • 02:11Preparing the Voltage-Sensitive Dye Solution
  • 03:18Preparing the Stomatogastric Ganglion and the Extracellular Recording
  • 03:58Dye Loading by Intracellular Injection
  • 06:35Imaging of Dyed Neurons
  • 08:03Results: Optical and Electrical Recordings from STG Neurons
  • 08:57Conclusion

Summary

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Here we present the methodology for fast and high resolution fluorescent voltage-sensitive dye imaging of detailed activity of neurons in the crab stomatogastric ganglion.

Tags

Optical ImagingNeuronsCrab Stomatogastric GanglionVoltage-sensitive DyesMethodologyNeuronal NetworksSimultaneous ActivityIntegral FunctionalityNetwork OrganizationPattern Generating NeuronsFluorescent Voltage-sensitive DyeDi-8-ANEPPQMiCAM02 High Speed And High Resolution CCD Camera Imaging SystemBVAna Imaging SoftwareElectrophysiology TechniquesCentral Pattern Generator Neural Networks

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