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Clonogenic Assay: Adherent Cells
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Biologie
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Journal JoVE Biologie
Clonogenic Assay: Adherent Cells

Clonogenic Assay: Adherent Cells

DOI:
10.3791/2573-v

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05:30 min

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March 13, 2011

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, , , , ,
1Epigenomic Medicine, BakerIDI Heart and Diabetes Institute,The Alfred Medical Research and Education Precinct, 2Department of Pathology,The University of Melbourne, 3Epigenetics in Human Health and Disease, BakerIDI Heart and Diabetes Institute,The Alfred Medical Research and Education Precinct, 4Department of Anatomy and Cellular Biology,The University of Melbourne

Chapitres

  • 00:01Titre
  • 00:05Introduction
  • 01:11Cell Preparation
  • 01:44Irradiation
  • 03:30Plating
  • 03:51Fixing and Staining
  • 05:02Conclusion

Summary

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The applicability of the clonogenic assay for evaluating reproductive viability has been established for more than 50 years. Here we demonstrate the general procedure for performing the clonogenic assay with adherent cells.

Tags

Clonogenic AssayColony Forming AssayReproductive ViabilityRadiation-dose Response CurveX-ray Irradiated CellsIonising RadiationCytotoxic AgentsGenetic ManipulationRadiation BiologyRadiation SensitivityRadiation Modifying CompoundsCytotoxic AgentsAnti-cancer TherapeuticsAdherent CellsCell MonolayerSingle Cell SuspensionsPetri DishesFixing And Staining Colonies

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