Journal
/
Biologie
/
Microarray Analysis for Saccharomyces cerevisiae
Journal JoVE
Biologie
This content is Free Access.
Journal JoVE Biologie
Microarray Analysis for Saccharomyces cerevisiae

Microarray Analysis for Saccharomyces cerevisiae

DOI:
10.3791/2585-v

•

13:17 min

•

April 07, 2011

•

, , ,
1Vermont Genetics Network,The University of Vermont

Chapitres

  • 00:05Titre
  • 02:18Extracting Total RNA from Yeast Cultures
  • 05:03Microarray Target Preparation
  • 09:44Results of Microarray Analysis
  • 10:56Conclusion

Summary

Traduction automatique

Traduction automatique

In this protocol, gene expression in yeast (Saccharomyces cerevisiae) is changed after exposure to oxidative stress induced by the addition of hydrogen peroxide (H2O2), an oxidizing agent.

Tags

Microarray AnalysisSaccharomyces CerevisiaeGene ExpressionOxidative StressHydrogen PeroxideYeast CultureGlucoseControl GroupTreated GroupHanks Buffered SalineTotal RNA ExtractionBiotin-labeled CRNAUVM Microarray Core FacilityAffymetrix Yeast GeneChipsGene Expression Data Analysis

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

check_url/fr/2585?article_type=v

Dissection of Saccharomyces Cerevisiae Asci
check_url/fr/2585?article_type=v

Quantitative Phosphoproteomics in Fatty Acid Stimulated Saccharomyces cerevisiae
check_url/fr/2585?article_type=v

A Fluorescence Microscopy Assay for Monitoring Mitophagy in the Yeast Saccharomyces cerevisiae
check_url/fr/2585?article_type=v

Visualization and Analysis of mRNA Molecules Using Fluorescence In Situ Hybridization in Saccharomyces cerevisiae
check_url/fr/2585?article_type=v

Coupled Assays for Monitoring Protein Refolding in Saccharomyces cerevisiae
check_url/fr/2585?article_type=v

EPR Monitored Redox Titration of the Cofactors of Saccharomyces cerevisiae Nar1
check_url/fr/2585?article_type=v

Rapid Identification of Chemical Genetic Interactions in Saccharomyces cerevisiae
check_url/fr/2585?article_type=v

Growth-based Determination and Biochemical Confirmation of Genetic Requirements for Protein Degradation in Saccharomyces cerevisiae
check_url/fr/2585?article_type=v

Directed Evolution Method in Saccharomyces cerevisiae: Mutant Library Creation and Screening
check_url/fr/2585?article_type=v

Cycloheximide Chase Analysis of Protein Degradation in Saccharomyces cerevisiae

Read Article