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Rejection of Fluorescence Background in Resonance and Spontaneous Raman Microspectroscopy
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Chapitres
- 00:05Titre
- 06:26Preparation of the Pump Beam (Kerr Beam)
- 07:46Setting Up the Kerr Gate and Collection System to Maximize the Collected Time-gated Signal
- 11:14Collecting a Time-gated Spectrum
- 12:31Representative Time-gated Raman Spectra
- 14:01Conclusion
We discuss the construction and operation of a complex nonlinear optical system that uses ultrafast all-optical switching to isolate Raman from fluorescence signals. Using this system we are able to successfully separate Raman and fluorescence signals utilizing pulse energies and average powers that remain biologically safe.
Tags
Fluorescence BackgroundRaman SpectroscopyBiological SamplesUltrafast PulsesPicosecond TimescaleRaman Scattered LightFluorescence SignalsNonlinear Optical SystemOptical Kerr GateLaser PowerRepetition RateNonlinear MediumAll-optical ShutterPeak EfficiencyPulse EnergiesAverage PowersBiologically SafeOptical Power
