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Modified Annexin V/Propidium Iodide Apoptosis Assay For Accurate Assessment of Cell Death
Journal JoVE
Biologie
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Journal JoVE Biologie
Modified Annexin V/Propidium Iodide Apoptosis Assay For Accurate Assessment of Cell Death

Modified Annexin V/Propidium Iodide Apoptosis Assay For Accurate Assessment of Cell Death

DOI:
10.3791/2597-v

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07:34 min

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April 24, 2011

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, , ,
1Department of Biological Sciences,University of Alberta, 2Department of Agriculture, Food and Nutrition Sciences,University of Alberta

Chapitres

  • 00:05Titre
  • 01:34Cell Preparation
  • 02:14Application of Annexin V/PI Stain
  • 04:26RNase-treated Cells and Cell Lines Exhibit Fewer False Positive Staining Results
  • 06:56Conclusion

Summary

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An accurate method for the assessment of cell death is described. The protocol improves upon conventional Annexin V/ propidium iodide (PI) protocols, which display up to 40% false- positive events in cell lines and primary cells from a broad range of animal models.

Tags

Annexin V/Propidium Iodide Apoptosis AssayCell Death StudiesFlow Cytometry-based MethodsPlasma Membrane IntegrityPermeabilityCell ViabilityNuclear StainsLate Apoptotic CellsNecrotic CellsFalse Positive EventsRNA StainingCytoplasmic Compartment

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