Journal
/
Biologie
/
Whole-mount Immunohistochemical Analysis for Embryonic Limb Skin Vasculature: a Model System to Study Vascular Branching Morphogenesis in Embryo
Journal JoVE
Biologie
Un abonnement à JoVE est nécessaire pour voir ce contenu.  Connectez-vous ou commencez votre essai gratuit.
Journal JoVE Biologie
Whole-mount Immunohistochemical Analysis for Embryonic Limb Skin Vasculature: a Model System to Study Vascular Branching Morphogenesis in Embryo

Whole-mount Immunohistochemical Analysis for Embryonic Limb Skin Vasculature: a Model System to Study Vascular Branching Morphogenesis in Embryo

DOI:
10.3791/2620-v

•

09:53 min

•

May 20, 2011

•

,
1Laboratory of Stem Cell and Neuro-Vascular Biology, Genetics and Developmental Biology Center,National Heart, Lung, and Blood Institute, National Institutes of Health

Chapitres

  • 00:05Titre
  • 01:42Collecting Embryonic Limb Skins
  • 03:53Whole-mount Immunohistochemical Staining of Limb Skins
  • 07:14Mounting the Limb Skins on a Slide
  • 08:24Results of Whole-mount Confocal Immunofluorescence Microscopy
  • 09:29Conclusion

Summary

Traduction automatique

Traduction automatique

We introduce a whole-mount immunohistochemistry and laser scanning confocal microscopy with multiple labelling for analyzing intricate vascular network formation in mouse embryonic limb skin.

Tags

Whole-mount Immunohistochemical AnalysisEmbryonic Limb Skin VasculatureVascular Branching MorphogenesisVascular DevelopmentLimb Skin Vasculature ModelWhole-mount Confocal MicroscopyFluorescent MarkersGenetic StudiesMolecular MechanismsPeripheral NervesSpatial TemplateDifferentiation And Patterning Of ArteriesStaining Intact Blood VesselsVascular Specific AntibodiesFluorescent Secondary AntibodiesVascularized Embryonic Organs

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

check_url/fr/2620?article_type=v

Double Whole Mount in situ Hybridization of Early Chick Embryos
check_url/fr/2620?article_type=v

Method for Whole Mount Antibody Staining in Chick
check_url/fr/2620?article_type=v

Zebrafish Whole Mount High-Resolution Double Fluorescent In Situ Hybridization
check_url/fr/2620?article_type=v

Mouse Embryonic Lung Culture, A System to Evaluate the Molecular Mechanisms of Branching
check_url/fr/2620?article_type=v

Whole Mount in Situ Hybridization of E8.5 to E11.5 Mouse Embryos
check_url/fr/2620?article_type=v

Do-It-Yourself Device for Recovery of Cryopreserved Samples Accidentally Dropped into Cryogenic Storage Tanks
check_url/fr/2620?article_type=v

A System for ex vivo Culturing of Embryonic Pancreas
check_url/fr/2620?article_type=v

Mouse Embryonic Development in a Serum-free Whole Embryo Culture System
check_url/fr/2620?article_type=v

Using Caenorhabditis elegans as a Model System to Study Protein Homeostasis in a Multicellular Organism
check_url/fr/2620?article_type=v

Analysis of Apoptosis in Zebrafish Embryos by Whole-mount Immunofluorescence to Detect Activated Caspase 3

Read Article