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全安装胚胎肢体皮肤血管的免疫组织化学分析：一个模型系统，研究在胚胎血管分支形态发生

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Biologie

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Journal JoVE Biologie

Whole-mount Immunohistochemical Analysis for Embryonic Limb Skin Vasculature: a Model System to Study Vascular Branching Morphogenesis in Embryo

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全安装胚胎肢体皮肤血管的免疫组织化学分析：一个模型系统，研究在胚胎血管分支形态发生

DOI:

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09:53 min

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May 20, 2011

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Wenling Li, Yoh-suke Mukouyama

¹Laboratory of Stem Cell and Neuro-Vascular Biology, Genetics and Developmental Biology Center,National Heart, Lung, and Blood Institute, National Institutes of Health

Chapitres

00:05Titre
01:42Collecting Embryonic Limb Skins
03:53Whole-mount Immunohistochemical Staining of Limb Skins
07:14Mounting the Limb Skins on a Slide
08:24Results of Whole-mount Confocal Immunofluorescence Microscopy
09:29Conclusion

Summary

Traduction automatique

我们介绍整个安装的免疫组织化学和激光扫描共聚焦显微镜分析复杂的小鼠胚胎肢体皮肤血管网的形成多个标签。

Tags

Whole-mount Immunohistochemical Analysis Embryonic Limb Skin Vasculature Vascular Branching Morphogenesis Vascular Development Limb Skin Vasculature Model Whole-mount Confocal Microscopy Fluorescent Markers Genetic Studies Molecular Mechanisms Peripheral Nerves Spatial Template Differentiation And Patterning Of Arteries Staining Intact Blood Vessels Vascular Specific Antibodies Fluorescent Secondary Antibodies Vascularized Embryonic Organs

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