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Transcriptome Analysis of Single Cells
Journal JoVE
Neurosciences
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Journal JoVE Neurosciences
Transcriptome Analysis of Single Cells

Transcriptome Analysis of Single Cells

DOI:
10.3791/2634-v

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07:27 min

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April 25, 2011

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, ,
1Department of Pharmacology,University of Pennsylvania, 2The Penn Genome Frontiers Institute,University of Pennsylvania

Chapitres

  • 00:05Titre
  • 01:11Preparing Culture and Pipettes
  • 01:53Harvesting Cells
  • 05:04Saving the Cell
  • 06:07Representative Results for Single Cell Harvest and aRNA Analysis
  • 07:08Conclusion

Summary

Traduction automatique

Traduction automatique

In this article we describe a simple method for the harvesting of single cells from rat primary neuronal cultures and subsequent transcriptome analysis using aRNA amplification. This approach is generalizable to any cell type.

Tags

Transcriptome AnalysisSingle CellsGene Expression AnalysisHeterogeneous PopulationsCell CultureHippocampusMRNA ProfilesMolecular DifferencesCell PopulationsHarvesting CellsElectrophysiologyEppendorf TubeMolecular Biology TechniquesARNA AmplificationDownstream ApplicationReverse-transcriptionReal-time Polymerase Chain Reaction (PCR)Linear AmplificationPolyadenylated RNA

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