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分离果蝇睾丸
Journal JoVE
Biologie
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Journal JoVE Biologie
Isolation of Drosophila melanogaster Testes
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分离果蝇睾丸

DOI:
10.3791/2641-v

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03:13 min

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May 13, 2011

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1Department of Biochemistry & Molecular Pharmacology and Howard Hughes Medical Institute,University of Massachusetts Medical School

Chapitres

  • 00:05Titre
  • 00:54Isolation of Drosophila Testes using Dissecting Needles
  • 02:44Conclusion

Summary

Traduction automatique

Traduction automatique

果蝇睾丸可迅速和有效地从成年男性使用解剖针分离。通过实践，人们可以很容易地在一至两天隔离睾丸足够的抗体或酶为基础的分析的高通量测序或更传统的分子生物学方法分析的DNA或RNA或蛋白质的量。

Tags

Drosophila Melanogaster TestesStem Cell MaintenanceDifferentiationMeiosisSoma-germline InteractionsTestes IsolationAdult MalesEclosionPupal CaseWild-type FliesWhite Mutant FliesGenetic BackgroundLaboratory ExperimentsGlass Microscope SlideBlack BackgroundIdentifying TestesDissecting NeedlesForcepsQuicker Method200-300 Wild-type Flies Per Hour400-600 Testes YieldTestes Size Reduction200-400 Testes Per Hour

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