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夹层和小鼠的外周淋巴结的双光子成像
Journal JoVE
Biologie
This content is Free Access.
Journal JoVE Biologie
Dissection and 2-Photon Imaging of Peripheral Lymph Nodes in Mice
DOI:

16:48 min

August 23, 2007

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Chapitres

  • 00:00Titre
  • 00:13Introduction
  • 00:41Tail Vein Injection for Adoptive Transfer of CFSE-labelled T Lympho
  • 03:45Removal of Lymph Nodes
  • 06:42Removing the Inguinal Lymph Nodes
  • 08:48Removing the Axillary Lymph Nodes
  • 11:24Removing the Cervical Lymph Nodes
  • 13:07Preparation of Lymph Nodes for In Situ Imaging
  • 15:362-photon Imaging of Lymph Nodes in Situ

Summary

Traduction automatique

双光子成像内已发现的基础条件下的淋巴结和淋巴细胞的活力和细胞相互作用过程中的免疫反应1。在这里,我们证明收养的T细胞,淋巴结的隔离和成像蠕动的CD4 + T细胞在explanted淋巴结转移。

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