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Chapitres
- 00:05Titre
- 01:27Phosphorus Extraction and pH Adjustment
- 02:13Enzyme Stock Preparation
- 02:59P Calibration Curve and Controls
- 04:23Sample + Enzyme Incubation
- 05:20Colorimetric Measurement of Released and Background Inorganic P
- 06:09Classification of P Compounds
- 07:35HTP Analysis of Phosphorus in Soil
- 08:26Conclusion
यह प्रोटोकॉल उच्च throughput एंजाइमी hydrolysis है कि एक microplate पाठक के इस्तेमाल को मापने के लिए और 96 के नमूने के पी monoesters, पी diesters और अकार्बनिक पी. के रूप में मिट्टी फास्फोरस एक मानक प्रयोगशाला में एक समय पर मापा जा सकता है है वर्गीकृत करने की एक विधि का वर्णन करता है.
Tags
High-throughput MeasurementClassificationOrganic PhosphorusEnvironmental SamplesOrganic P CompoundsTraditional P MeasurementsColorimetric ReagentsEnzymatic HydrolysisAccurate CharacterizationP FormsPhosphorus-31 Nuclear Magnetic Resonance Spectroscopy31P-NMRExpensive MethodSpecialized Technical TrainingAdapted Enzymatic Hydrolysis MethodMicroplate Reader SystemFast And Accurate MeasurementAffordable MethodUser-friendly MeansP Species In SoilsSedimentsManuresAquatic SamplesHigh-throughput MethodForms And Enzyme-lability Of Organic PStandard LaboratorySystem Nutrient ContentEutrophication Potential
