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Thermodynamics of Membrane Protein Folding Measured by Fluorescence Spectroscopy
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Journal JoVE Bio-ingénierie
Thermodynamics of Membrane Protein Folding Measured by Fluorescence Spectroscopy

Thermodynamics of Membrane Protein Folding Measured by Fluorescence Spectroscopy

DOI:
10.3791/2669-v

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10:09 min

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April 28, 2011

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1Chemistry and Biochemistry,University of California San Diego – UCSD

Chapitres

  • 00:05Titre
  • 00:44Introduction
  • 01:24Preparation of ~50 nm Diameter Small Unilamellar Vesicles (SUVs) for Membrane Protein Folding
  • 03:10Sample Preparation for Initial Fluorescence Unfolding Curve
  • 04:34Measurement of Fluorescence Spectra
  • 05:45Generation of Initial Unfolding Curve and Estimation of Gibbs Free Energy of Membrane Protein Unfolding
  • 08:21Representative Results for the Measurement of Membrane Protein Unfolding
  • 09:22Conclusion

Summary

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Traduction automatique

This video article details the experimental procedure for obtaining the Gibbs free energy of membrane protein folding by tryptophan fluorescence.

Tags

ThermodynamicsMembrane Protein FoldingFluorescence SpectroscopyGibbs Free EnergyUnfoldingDenaturantIntegral Membrane ProteinE. ColiEquilibrium PopulationsFolded StatesUnfolded StatesDenaturant ConcentrationLipid VesiclesData Analysis ProcedureTemperaturePHFolding EnvironmentsLipidsMicelles

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