/

/

天堂斑马鱼的细胞标记与激光Uncagable荧光葡聚糖

Journal JoVE
Biologie

Un abonnement à JoVE est nécessaire pour voir ce contenu. Connectez-vous ou commencez votre essai gratuit.

Journal JoVE Biologie

Lineage Labeling of Zebrafish Cells with Laser Uncagable Fluorescein Dextran

Please note that all translations are automatically generated. Click here for the English version.

天堂斑马鱼的细胞标记与激光Uncagable荧光葡聚糖

DOI:

•

07:35 min

•

April 28, 2011

•

Joshua A. Clanton, Ilya A. Shestopalov, James K. Chen, Joshua T. Gamse

¹Department of Biological Sciences,Vanderbilt University, ²Department of Chemical and Systems Biology,Stanford University

Chapitres

00:05Titre
01:05Injection of Caged Fluorescein Dextran into Zebrafish Embryos
02:49Laser Uncaging of Fluorescein Dextran
04:14Antibody Labeling and Detection of Uncaged Fluorescein Dextran
06:36Representative Immunolabeling Results
07:19Conclusion

Summary

Traduction automatique

该协议描绘的方式，标签和跟踪的小斑马鱼的胚胎细胞群体，使用紫外线uncaging笼荧光，整个装载免疫标记uncaged荧光信号放大的命运。

Tags

Lineage Labeling Zebrafish Cells Laser Uncagable Fluorescein Dextran Biologie du développement Cell Types Lineage Labeling Methods DiI Labeling Traceable Enzymes Zebrafish Labs Photoconvertable Fluorescent Proteins Eos Kaede Ultraviolet Light Transgenesis Dye-mediated Lineage Labeling Methods

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

与非侵入性检测荧光染料标记的干细胞与光学成像

在斑马鱼胚胎的代谢标记和Bioorthogonal点击化学成像聚糖

激光烧蚀斑马鱼Pronephros研究肾小管上皮再生

表型分析和分离小鼠造血干细胞和传承的承诺祖

代谢标记和流式细胞仪测定细胞周期进程的动力学

激光伤害的斑马鱼胚胎骨骼肌

肾毒素显微注射斑马鱼到模型急性肾损伤

连续波ium激光加热培养细胞以研究细胞热效应

带谱系特异标记的间充质细胞脂肪分化电位的可视化与量化

使用双标记（MADM）的马赛克分析开发脑皮质的血统跟踪和克隆分析

Read Article