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Lineage Labeling of Zebrafish Cells with Laser Uncagable Fluorescein Dextran
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Journal JoVE Biologie
Lineage Labeling of Zebrafish Cells with Laser Uncagable Fluorescein Dextran

Lineage Labeling of Zebrafish Cells with Laser Uncagable Fluorescein Dextran

DOI:
10.3791/2672-v

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07:35 min

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April 28, 2011

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, , ,
1Department of Biological Sciences,Vanderbilt University, 2Department of Chemical and Systems Biology,Stanford University

Chapitres

  • 00:05Titre
  • 01:05Injection of Caged Fluorescein Dextran into Zebrafish Embryos
  • 02:49Laser Uncaging of Fluorescein Dextran
  • 04:14Antibody Labeling and Detection of Uncaged Fluorescein Dextran
  • 06:36Representative Immunolabeling Results
  • 07:19Conclusion

Summary

Traduction automatique

Traduction automatique

This protocol delineates a way to label and trace the fate of small groups of cells zebrafish embryos using UV-uncaging of caged fluorescein, followed by whole mount immunolabeling to amplify the signal from the uncaged fluorescein.

Tags

Lineage LabelingZebrafish CellsLaser Uncagable Fluorescein DextranBiologie du développementCell TypesLineage Labeling MethodsDiI LabelingTraceable EnzymesZebrafish LabsPhotoconvertable Fluorescent ProteinsEosKaedeUltraviolet LightTransgenesisDye-mediated Lineage Labeling Methods

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